Index to Chiropractic Literature
Index to Chiropractic Literature
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Friday, September 30, 2022
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ID 24987
  Title TENS-like stimulation downregulates inflammatory cytokines in a PC-12 cell line
URL https://www.ncbi.nlm.nih.gov/pubmed/28822472
Journal J Manipulative Physiol Ther. 2017 Jul-Aug;40(6):381-386
Author(s)
Subject(s)
Peer Review Yes
Publication Type Article
Abstract/Notes

Objectives: The purpose of this study was to evaluate the effects of transcutaneous electrical nerve stimulation (TENS)–like stimulation on the expression of the proinflammatory cytokines tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-6 in PC-12 cells, which are commonly used as neuronal cell models.

Methods: Nerve growth factor–differentiated PC-12 cells were exposed to electrical stimulation for 15 minutes at 1 mA, 200 μs, and 100 Hz. Cell lysate from stimulated and control cells was assayed for TNF-α, IL-1β, and IL-6. In 6 trials, cells were preincubated with the L-type ion channel blocker nicardipine. Cultured cells were also incubated with Alexa Fluor 488 and visualized by fluorescence microscopy to determine the nuclear vs cytoplasmic distribution of the p65 sub-unit of NF-κB

Results: Compared with control (unstimulated) cells, the stimulated cells had a downregulation of the assayed cytokines. However, preincubation with the L-type ion channel blocker nicardipine blocked this effect of stimulation. Additionally, it was noted that TENS-like stimulation promoted a relative sequestration of the p65 subunit of NF-κB in the cytoplasm vs the nucleus.

Conclusions: It appears that in this cell line and with these stimulation parameters, TENS-like stimulation attenuated the expression of the assayed proinflammatory cytokines, in part by promoting the relative sequestration of the p65 subunit of NF-κB in the cytoplasm, and that voltage-dependent calcium channels have a role in the cascade of events initiated by the TENS-like stimulation.

Author keywords: Cytokines; Transcutaneous Electrical Nerve Stimulation

This abstract is reproduced with the permission of the publisher; full text is available by subscription. Click on the above link and select a publisher from PubMed's LinkOut feature.


 

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